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KMID : 0380020110260050459
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Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 5 p.459 ~ p.464
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Purification of Peat Moss Extract Using a Supercritical CO 2 and Verification of Its Biological Activities
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Kim Mi-Hyang
Jeon Myong-Je
Park Mi-Ra
Lee Seung-Woo
Hwang Hyun-Jung
Jang Hye-Ji
Kang Min-Suk
Kim Bo-Kyung
Choi Seung-Tae
Park Mi-Yeon
Lee Sang-Hyeon
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Abstract
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In this study, the canadian peat moss extract was purified by a supercritical CO2 using three different conditions and assessed its biological activities. Peat moss was extracted by acid-alkaline extraction method (sample 1) and purified by a supercritical CO2 at 40℃ under pressure of 100 bar (sample 2), 120 bar (sample 3) or 150 bar (sample 4). We evaluated the antioxidant activities of the samples by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging, Fe2+/ascorbate (FTC) and 2-thiobarbituric acid (TBA) methods. The antioxidant activities were examined by comparing the results with that of ascorbic acid as a positive control. Sample 3 showed relatively higher DPPH radical-scavenging activities than other samples. The antioxidant activity by FIC method exhibited similar results as the DPPH radicalscavenging activities. On the other hand, sample 2 showed higher antioxidant activity measured by TBA method of all. The whitening effects of the samples were examined using mushroom tyrosinase and B16F10 melanoma cells. Sample 3 exhibited overall significant whitening effects, however, other samples showed relatively lower effects. These results suggest that the peat moss extract purified by a supercritical CO2 could be used as a cosmetic ingredient for the anti-aging and whitening effects.
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KEYWORD
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antioxidant activity, peat moss, supercritical 2, whitening effect
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